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Journal / Developmental Biology / Use of Immunolabeling to Analyze Stable, Dynamic, …
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Developmental Biology

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JoVE Journal Developmental Biology
Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
 

Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo

Article DOI: 10.3791/55792-v • 12:38 min • September 20th, 2017
September 20th, 2017 •
*1, *1, 1, 1, 1
1Department of Biological Sciences, University of Maryland, Baltimore County
* These authors contributed equally

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Immunolabeling methods to analyze distinct populations of microtubules in the developing zebrafish brain are described here, which are broadly applicable to other tissues. The first protocol outlines an optimized method for immunolabeling stable and dynamic microtubules. The second protocol provides a method to image and quantify nascent microtubules specifically.

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Immunolabeling Microtubules Zebrafish Embryo Stable Microtubules Dynamic Microtubules Nascent Microtubules Developmental Neurobiology Cell Polarity Detection Quantification In Situ Microtubule Integrity De-chlorinating Zebrafish Embryos Fixation Microtubule Assembly Buffer MAB PFA TBS NP-40 Buffer Wash Agarose Embedding
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