/
/
Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
8,138 Views
•
12:38 min
•
September 20, 2017
DOI:
Summary
Automatically generated
Immunolabeling methods to analyze distinct populations of microtubules in the developing zebrafish brain are described here, which are broadly applicable to other tissues. The first protocol outlines an optimized method for immunolabeling stable and dynamic microtubules. The second protocol provides a method to image and quantify nascent microtubules specifically.
Read Article
Cite this Article
McFarland, R. J., Brown, S. P., Vital, E., Werner, J. M., Brewster, R. M. Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo. J. Vis. Exp. (127), e55792, doi:10.3791/55792 (2017).
Copy