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Análise de Microglia e Macropagos derivados de Monócitos do Sistema Nervoso Central por Citometria de Fluxo
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Immunology and Infection
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JoVE Journal Immunology and Infection
Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

Análise de Microglia e Macropagos derivados de Monócitos do Sistema Nervoso Central por Citometria de Fluxo

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22,799 Views

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10:43 min

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June 22, 2017

DOI:

10.3791/55781-v

DOI:
10.3791/55781-v

•

10:43 min
June 22, 2017

•

25 Views
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1Inserm U 1227, CNRS UMR 7225, 2Sorbonne Universités,UPMC, University of Paris, 3Institut du Cerveau et de la Moelle épinière, ICM, 4AP-HP, Hôpital de la Pitié Salpêtrière;

Summary

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Este protocolo fornece uma análise das subpopulações de macrófagos no sistema nervoso central do mouse adulto por citometria de fluxo e é útil para o estudo de marcadores múltiplos expressos por essas células.

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Martin, E., El-Behi, M., Fontaine, B., Delarasse, C. Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry. J. Vis. Exp. (124), e55781, doi:10.3791/55781 (2017).

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