Journal
/
Immunologie und Infektion
/
Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

DOI:
10.3791/55781-v

•

10:43 min

•

June 22, 2017

•

, , ,
1Inserm U 1227, CNRS UMR 7225, 2Sorbonne Universités,UPMC, University of Paris, 3Institut du Cerveau et de la Moelle épinière, ICM, 4AP-HP, Hôpital de la Pitié Salpêtrière;

Kapitel

  • 00:05Titel
  • 00:52Perfusion and Brain and Spinal Cord Dissection
  • 03:18Cell Dissociation and Density Gradient Separation
  • 06:00Cell Labeling for Flow Cytometry
  • 08:06Results: Representative Brain and Spinal Cord Macrophage Subpopulation Analysis
  • 09:31Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol provides an analysis of the macrophage subpopulations in the adult mouse central nervous system by flow cytometry and is helpful for the study of multiple markers expressed by these cells.

Tags

MicrogliaMonocyte-derived MacrophagesCentral Nervous SystemFlow CytometryNeuroimmunologyMacrophage SubpopulationsCell Marker AnalysisCell IsolationSpinal CordBrain

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Culturing Microglia from the Neonatal and Adult Central Nervous System

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells

Read Article