Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

Elodie Martin; Mohamed El-Behi; Bertrand Fontaine; Cecile Delarasse

doi:10.3791/55781

Journal

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Immunologie und Infektion

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Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

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Immunologie und Infektion

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JoVE Journal Immunologie und Infektion

Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

22,799 Views

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10:43 min

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June 22, 2017

DOI:

10.3791/55781-v

DOI:

10.3791/55781-v

•

10:43 min

June 22, 2017

•

25 Views

Elodie Martin^1,2,3, Mohamed El-Behi^1,2,3, Bertrand Fontaine^1,2,3,4, Cecile Delarasse^1,2,3

¹Inserm U 1227, CNRS UMR 7225, ²Sorbonne Universités,UPMC, University of Paris, ³Institut du Cerveau et de la Moelle épinière, ICM, ⁴AP-HP, Hôpital de la Pitié Salpêtrière;

Summary

This protocol provides an analysis of the macrophage subpopulations in the adult mouse central nervous system by flow cytometry and is helpful for the study of multiple markers expressed by these cells.

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Microglia Monocyte-derived Macrophages Central Nervous System Flow Cytometry Neuroimmunology Macrophage Subpopulations Cell Marker Analysis Cell Isolation Spinal Cord Brain

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Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

Summary

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