JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Journal
/
Genetics
/
通过两个互补细胞同步方案研究细胞周期调控的基因表达
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Genetics
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Please note that all translations are automatically generated.
Click here for the English version.
通过两个互补细胞同步方案研究细胞周期调控的基因表达
DOI:
10.3791/55745-v
•
12:02 min
•
June 06, 2017
•
Aintzane Apraiz*
1
,
Jone Mitxelena*
2,3
,
Ana Zubiaga
1
Department of Cell Biology and Histology
,
University of the Basque Country, UPV/EHU
,
2
Department of Genetics, Physical Anthropology and Animal Physiology
,
University of the Basque Country, UPV/EHU
,
3
Department of Molecular Mechanisms of Disease
,
University of Zurich
Chapters
00:05
Title
00:41
Thymidine- and Nocodazole-based (Thy-noc) Synchronization and Release
03:34
HU-based Synchronization and Release from G1/S Boundary
05:14
Collecting Samples for FACS Analysis and RNA Extraction
07:00
RNA Extraction for Gene Expression Analysis
09:02
Results: Thy-noc and HU Cell Synchronization Protocols Enable Analysis of Gene Expression During Specific Phases of the Cell Cycle
11:07
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们报告两个细胞同步协议,提供一个上下文,用于研究与细胞周期的特定阶段相关的事件。我们表明,这种方法可用于分析未受扰动细胞周期中特定基因的调节,或暴露于影响细胞周期的试剂。
Tags
Cell Cycle
Gene Expression
Cell Synchronization
U2OS Cells
Thymidine Block
Nocodazole
Mitotic Cell Arrest
G2-M
RNA Extraction
FACS Analysis
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
在人原代细胞培养生物钟基因表达及荷尔蒙分泌的并行测量
单细胞基因表达利用多重RT-qPCR的表征罕见的淋巴人群的异质性
饲养并在隐藏甲壳虫双链RNA介导的基因敲除,<em>白腹皮蠹</em
单细胞基因表达谱使用流式细胞仪和qPCR内部标准
选择依赖和独立生成CRISPR / Cas9介导的哺乳动物细胞基因敲除
逆转录病毒扫描:映射MLV集成站点以定义细胞特异性监管区域
量化的信息编码的基因表达水平在寿命调制下
线虫
的宽范围饮食限制
细胞间相互作用中基因表达模式的控制与分析的 Optogenetic 方法
CRISPR/Cas9 对小鼠和人造血祖细胞的高效基因破坏
从土壤、根际和根 Endosphere 提取细菌群落数据的根菌群探析
Read Article