Journal
/
Genetik
/
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
JoVE Journal
Genetik
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Genetik
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

26,660 Views

•

12:02 min

•

June 06, 2017

DOI:

10.3791/55745-v

DOI:
10.3791/55745-v

•

12:02 min
June 06, 2017

•

11 Views
, ,
1Department of Cell Biology and Histology,University of the Basque Country, UPV/EHU, 2Department of Genetics, Physical Anthropology and Animal Physiology,University of the Basque Country, UPV/EHU, 3Department of Molecular Mechanisms of Disease,University of Zurich

Summary

Automatically generated

We report two cell synchronization protocols that provide a context for studying events related to specific phases of the cell cycle. We show that this approach is useful for analyzing the regulation of specific genes in an unperturbed cell cycle or upon exposure to agents affecting the cell cycle.

Explore More Videos

Cell CycleGene ExpressionCell SynchronizationU2OS CellsThymidine BlockNocodazoleMitotic Cell ArrestG2-MRNA ExtractionFACS Analysis

Read Article

Cite this Article

Apraiz, A., Mitxelena, J., Zubiaga, A. Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols. J. Vis. Exp. (124), e55745, doi:10.3791/55745 (2017).

Download .ris file

Copy

Share Video

.

Copy