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胚胎血管生成的可视化和定量分析<em>热带非洲爪蟾</em
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Developmental Biology
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JoVE Journal Developmental Biology
Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis
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胚胎血管生成的可视化和定量分析<em>热带非洲爪蟾</em

DOI:
10.3791/55652-v

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06:05 min

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May 25, 2017

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1Brain Korea 21 PLUS Project for Medical Science,Yonsei University College of Medicine, 2Department of Anatomy, Brain Research Institute,Yonsei University College of Medicine

Chapters

  • 00:05Title
  • 00:38Human Plasma Acetylated Low Density Lipoprotein Dil Complex (Dil-AcLDL) Injection Preparation
  • 02:27Dil-AcLDL Injection and Xenopus tropicalis Embryo Fixation
  • 03:44Results: Representative Xenopus tropicalis Imaging
  • 05:02Conclusion

Summary

Automatic Translation

Automatic Translation

该方案证明了一种基于荧光的方法来显示脉管系统并量化其在热带非洲爪蟾中的复杂性 。在遗传和/或药理学操作之后，将荧光染料注射到胚胎的跳动心脏中以在体内研究心血管发育后几分钟可以对血管进行成像。

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