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Le reti neurali ricalcolate anatomicamente tridimensionali dei micro-tessuti per la ricostruzione, la modulazione e la modellazione del sistema nervoso
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1Department of Bioengineering, School of Engineering and Applied Science,University of Pennsylvania, 2Center for Brain Injury & Repair, Department of Neurosurgery, Perelman School of Medicine,University of Pennsylvania, 3Center for Neurotrauma, Neurodegeneration & Restoration,Michael J. Crescenz Veterans Affairs Medical Center, 4School of Biomedical Engineering,Drexel University
Chapters
- 00:05Title
- 00:58Micro-column Fabrication Using Capillary Tubes
- 02:04Micro-column Trimming and Sterilization
- 02:48Preparation of the Pyramidal Micro-well Array
- 04:46Formation of Neuronal Aggretates
- 05:27ECM Core Fabrication
- 06:42Neuronal Aggregate Seeding of Micro-columns
- 08:14Results: Observation of Axonal Growth in Micro-TENNs by Phase-contrast Imaging and Cytoarchitecture by Confocal Microscopy
- 09:51Conclusion
Questo manoscritto specifica la realizzazione di reti neurali rivelate micro-tessuto: costruzioni tridimensionali di dimensioni microniche costituite da tratti longitudinali allineati allineati che comprendono la popolazione neuronale aggregata racchiuse in un idrogel tubolare. Questi impalcature viventi possono servire come relè funzionali per ricostruire o modulare la circuiteria neurale o come test-letti biofidelici che imitano la neuroanatomia della materia grigio-bianca.
