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Een Chronisch Autoimmuun Droog Oog Rat Model Met Toename In Effector Geheugen T Cellen In Oogappel Weefsel
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
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Een Chronisch Autoimmuun Droog Oog Rat Model Met Toename In Effector Geheugen T Cellen In Oogappel Weefsel

Nederlands

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11,181 Views

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09:42 min

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June 07, 2017

DOI:

10.3791/55592-v

DOI:
10.3791/55592-v

•

09:42 min
June 07, 2017

•

6 Views
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1Singapore Eye Research Insitute, A Member of SingHealth, 2DUKE-National University of Singapore Medical School, 3Lee Kong Chian School of Medicine,Nanyang Technological University, 4Singapore National Eye Center, 5Yong Loo Lin School of Medicine,National University of Singapore

Summary

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Dit rapport beschrijft een methode om chronische experimentele auto-immuun droge ogen in Lewis-ratten te induceren door middel van immunisatie met een emulsie van ratlakakalextract, ovalbumine en complete Freund's adjuvans, gevolgd door de injectie van lacrimale klier extract en ovalbumine in de forniceal subconjunctiva en lacrimale klieren Zes weken later.

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Autoimmune Dry EyeRat ModelLacrimal GlandEffector Memory T CellsOcular DiseaseLymphocyte TraffickingSprague Dawley RatLacrimal Gland ExtractFreund’s AdjuvantOvalbumin

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Cite this Article

Hou, A., Bose, T., Chandy, K. G., Tong, L. A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue. J. Vis. Exp. (124), e55592, doi:10.3791/55592 (2017).

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