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A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
DOI:

09:42 min

June 07, 2017

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Kapitel

  • 00:05Titel
  • 01:18Preparation of Lacrimal Gland Extract
  • 02:32Preparation of Emulsion
  • 03:30Immunization of Lewis Rats and Injection of Antigen Mixture into the Forniceal Subconjunctiva and Lacrimal Gland
  • 04:53Assessment of Dry Eye Features
  • 07:19Ergebnisse
  • 08:57Conclusion

Summary

Automatische Übersetzung

This report describes a method to induce chronic experimental autoimmune dry eye in Lewis rats through immunization with an emulsion of rat lacrimal gland extract, ovalbumin, and complete Freund's adjuvant, followed by the injection of lacrimal gland extract and ovalbumin into the forniceal subconjunctiva and lacrimal glands six weeks later.

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