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A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue

A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue

11,197 Views

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09:42 min

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June 07, 2017

DOI:

10.3791/55592-v

DOI:
10.3791/55592-v

•

09:42 min
June 07, 2017

•

6 Views
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1Singapore Eye Research Insitute, A Member of SingHealth, 2DUKE-National University of Singapore Medical School, 3Lee Kong Chian School of Medicine,Nanyang Technological University, 4Singapore National Eye Center, 5Yong Loo Lin School of Medicine,National University of Singapore

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This report describes a method to induce chronic experimental autoimmune dry eye in Lewis rats through immunization with an emulsion of rat lacrimal gland extract, ovalbumin, and complete Freund's adjuvant, followed by the injection of lacrimal gland extract and ovalbumin into the forniceal subconjunctiva and lacrimal glands six weeks later.

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Autoimmune Dry EyeRat ModelLacrimal GlandEffector Memory T CellsOcular DiseaseLymphocyte TraffickingSprague Dawley RatLacrimal Gland ExtractFreund’s AdjuvantOvalbumin

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Hou, A., Bose, T., Chandy, K. G., Tong, L. A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue. J. Vis. Exp. (124), e55592, doi:10.3791/55592 (2017).

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