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通过高通量抑制剂筛选鉴定调节胚胎干细胞多能性的激酶的简单方法
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
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通过高通量抑制剂筛选鉴定调节胚胎干细胞多能性的激酶的简单方法

DOI:
10.3791/55515-v

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07:18 min

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May 12, 2017

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, ,
1The MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences,University of Dundee, 2Department of Cancer Biology,Dana-Farber Cancer Institute, 3Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School

Chapters

  • 00:05Title
  • 00:47mESC Culture Conditions and Procedures for Screen Preparation
  • 03:09Kinase Inhibitor Screening Analysis
  • 04:56Results: Identification of Kinase Inhibitors that Modulate mESC Pluripotency
  • 06:13Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们提出一个定量和可扩展的方案来执行针对性的小分子屏幕，用于初始化多能转换的激酶调节剂。

Tags

Embryonic Stem CellPluripotencyKinase Inhibitor ScreeningHigh-throughputNovel Signaling PathwaysMouse ES CellsGelatin CoatingCell CultureTrypsinizationCell Counting96-well PlateKinase Inhibitor Treatment

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