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A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

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07:18 min

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May 12, 2017

DOI:

10.3791/55515-v

DOI:
10.3791/55515-v

•

07:18 min
May 12, 2017

•

1 Views
, ,
1The MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences,University of Dundee, 2Department of Cancer Biology,Dana-Farber Cancer Institute, 3Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School

Summary

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Here, we present a quantitative and scalable protocol to perform targeted small molecule screens for kinase regulators of the naïve-primed pluripotent transition.

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Williams, C. A. C., Gray, N. S., Findlay, G. M. A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening. J. Vis. Exp. (123), e55515, doi:10.3791/55515 (2017).

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