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Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils
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Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils

Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils

8,386 Views

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09:32 min

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April 13, 2017

DOI:

10.3791/55503-v

DOI:
10.3791/55503-v

•

09:32 min
April 13, 2017

•

7 Views
, , , , ,
1German Center for Neurodegenerative Diseases (DZNE), 2Centre for Prions and Protein Folding Diseases & Department of Biochemistry,University of Alberta

Summary

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Peripheral injection of alpha-synuclein fibrils into the peritoneum or tongue of Tg(M83+/-:Gfap-luc+/-) mice, which express human alpha-synuclein with the familial A53T mutation and firefly luciferase, can induce neuropathology, including neuroinflammation, in their central nervous system.

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Bioluminescence ImagingNeuroinflammationTransgenic MiceAlpha-synuclein FibrilsParkinson’s DiseaseNoninvasive MonitoringAnesthesiaIntranasal InoculationIntraperitoneal InjectionNerve InflammationNeurological Disease

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Cite this Article

Breid, S., Bernis, M. E., Tachu, J. B., Garza, M. C., Wille, H., Tamgüney, G. Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils. J. Vis. Exp. (122), e55503, doi:10.3791/55503 (2017).

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