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酵素的に活性G4 Resolvase1の精製のためのG-四重鎖DNA親和性のアプローチ
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Biochemistry
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JoVE Journal Biochemistry
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
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酵素的に活性G4 Resolvase1の精製のためのG-四重鎖DNA親和性のアプローチ

日本語

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9,457 Views

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11:25 min

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March 18, 2017

DOI:

10.3791/55496-v

DOI:
10.3791/55496-v

•

11:25 min
March 18, 2017

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4 Views
, , , , ,
1Department of Cancer Biology,Wake Forest School of Medicine, 2YX Genomics, 3Department of Biology,Ball State University, 4Department of Hematology and Oncology,Roper St. Francis Hospital

Summary

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G4 Resolvase1はG4結合タンパク質のための最も厳しい報告親和性でG-四重鎖（G4）の構造に結合し、HeLa細胞におけるG4-DNA巻き戻し活性の大部分を表しています。我々は、具体的には、触媒活性組換えG4R1を精製するための親和性およびG4-Resolvase1のATP依存性巻き戻し活性を活かした新たなプロトコルを記述します。

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Cite this Article

Routh, E. D., Creacy, S. D., Beerbower, P. E., Akman, S. A., Vaughn, J. P., Smaldino, P. J. A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1. J. Vis. Exp. (121), e55496, doi:10.3791/55496 (2017).

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