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A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
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Biochemie
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JoVE Journal Biochemie
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

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11:25 min

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March 18, 2017

DOI:

10.3791/55496-v

DOI:
10.3791/55496-v

•

11:25 min
March 18, 2017

•

4 Views
, , , , ,
1Department of Cancer Biology,Wake Forest School of Medicine, 2YX Genomics, 3Department of Biology,Ball State University, 4Department of Hematology and Oncology,Roper St. Francis Hospital

Summary

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G4 Resolvase1 binds to G-quadruplex (G4) structures with the tightest reported affinity for a G4-binding protein and represents the majority of the G4-DNA unwinding activity in HeLa cells. We describe a novel protocol that harnesses the affinity and ATP-dependent unwinding activity of G4-Resolvase1 to specifically purify catalytically active recombinant G4R1.

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Routh, E. D., Creacy, S. D., Beerbower, P. E., Akman, S. A., Vaughn, J. P., Smaldino, P. J. A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1. J. Vis. Exp. (121), e55496, doi:10.3791/55496 (2017).

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