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Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
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Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
Capitoli
- 00:05Titolo
- 00:51Lung Harvest and Preparation
- 02:36Lung Slicing
- 04:29Static PCLS Imaging
- 05:44PCLS Live-cell Imaging
- 07:01Results: Representative Immune Cell Population Identification
- 08:36Conclusion
We describe a method for generating Precision-cut Lung Slices (PCLS) and immunostaining them to visualize the localization of various immune cell types in the lung. Our protocol can be extended to visualize the location and function of many different cell types under a variety of conditions.
