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Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
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Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
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April 05, 2017
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Summary
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We describe a method for generating Precision-cut Lung Slices (PCLS) and immunostaining them to visualize the localization of various immune cell types in the lung. Our protocol can be extended to visualize the location and function of many different cell types under a variety of conditions.
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Lyons-Cohen, M. R., Thomas, S. Y., Cook, D. N., Nakano, H. Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells. J. Vis. Exp. (122), e55465, doi:10.3791/55465 (2017).
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