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Aislamiento, caracterización y purificación de los macrófagos de los tejidos afectados por la inflamación relacionados con la obesidad
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
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Aislamiento, caracterización y purificación de los macrófagos de los tejidos afectados por la inflamación relacionados con la obesidad

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24,794 Views

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07:46 min

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April 03, 2017

DOI:

10.3791/55445-v

DOI:
10.3791/55445-v

•

07:46 min
April 03, 2017

•

8 Views
, , , , , , ,
1Department of Veterinary and Biomedical Sciences,The Pennsylvania State University, 2Microscopy and Cytometry Facility, The Huck Institutes of Life Sciences,Pennsylvania State University

Summary

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Este protocolo permite un investigador aislar y caracterizar tejido residente macrófagos en varios sello inflamado los tejidos extraídos de modelos inducida por la dieta de los trastornos metabólicos.

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Cite this Article

Allen, J. N., Dey, A., Nissly, R., Fraser, J., Yu, S., Balandaram, G., Peters, J. M., Hankey-Giblin, P. A. Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation. J. Vis. Exp. (122), e55445, doi:10.3791/55445 (2017).

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