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Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

24,771 Views

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07:46 min

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April 03, 2017

DOI:

10.3791/55445-v

DOI:
10.3791/55445-v

•

07:46 min
April 03, 2017

•

8 Views
, , , , , , ,
1Department of Veterinary and Biomedical Sciences,The Pennsylvania State University, 2Microscopy and Cytometry Facility, The Huck Institutes of Life Sciences,Pennsylvania State University

Summary

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This protocol allows a researcher to isolate and characterize tissue-resident macrophages in various hallmark inflamed tissues extracted from diet-induced models of metabolic disorders.

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Allen, J. N., Dey, A., Nissly, R., Fraser, J., Yu, S., Balandaram, G., Peters, J. M., Hankey-Giblin, P. A. Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation. J. Vis. Exp. (122), e55445, doi:10.3791/55445 (2017).

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