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Live-Imaging van antischimmelactiviteit door Human Primary neutrofielen en monocyten in reactie op<em> A. fumigatus</em
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus
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Live-Imaging van antischimmelactiviteit door Human Primary neutrofielen en monocyten in reactie op<em> A. fumigatus</em

Nederlands

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9,855 Views

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12:29 min

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April 19, 2017

DOI:

10.3791/55444-v

DOI:
10.3791/55444-v

•

12:29 min
April 19, 2017

•

2 Views
, , , , , ,
1Aberdeen Fungal Group, MRC Centre for Medical Mycology, Institute of Medical Sciences,University of Aberdeen, 2Department of Medicine,Memorial Sloan-Kettering Cancer Center, New York, US

Summary

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Hier beschrijven we een protocol om antifungale activiteit van primaire menselijke immuuncellen te beoordelen in real-time met behulp van fluorescente Aspergillus reporter conidia in combinatie met live-cell video microscopie en flowcytometrie. Gegenereerde data inzicht in gastheer Aspergillus cel-interacties zoals fungicide werking, fagocytose, celmigratie remming van de schimmelgroei.

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Live Cell ImagingAntifungal ActivityHuman NeutrophilsHuman MonocytesAspergillus FumigatusFluorescent Reporter ConidiaInnate Immune ResponsePhagocytosisCell MigrationFungal Growth Inhibition

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Cite this Article

Brunel, S. F., Bain, J. M., King, J., Heung, L. J., Kasahara, S., Hohl, T. M., Warris, A. Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus. J. Vis. Exp. (122), e55444, doi:10.3791/55444 (2017).

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