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Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus
JoVE Journal
İmmünoloji ve Enfeksiyon
This content is Free Access.
JoVE Journal İmmünoloji ve Enfeksiyon
Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus

Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus

DOI:
10.3791/55444-v

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12:29 min

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April 19, 2017

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, , , , , ,
1Aberdeen Fungal Group, MRC Centre for Medical Mycology, Institute of Medical Sciences,University of Aberdeen, 2Tıp Fakültesi,Memorial Sloan-Kettering Cancer Center, New York, US

Bölümler

  • 00:05Başlık
  • 00:54Aspergillus fumigatus Culture and Labeling
  • 04:28Isolation of Human Monocytes
  • 07:30Isolation of Human Neutrophils
  • 08:30Live Cell Video Microscopy
  • 09:31Results: In Vitro Evaluation of Primary Immune Response to Aspergillus Conidia
  • 11:11Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we describe a protocol to assess antifungal activity of primary human immune cells in real-time using fluorescent Aspergillus reporter conidia in conjunction with live-cell video microscopy and flow cytometry. Generated data provide insight into host cell-Aspergillus interactions such as fungicidal activity, phagocytosis, cell migration and inhibition of fungal growth.

Etiketler

Live Cell ImagingAntifungal ActivityHuman NeutrophilsHuman MonocytesAspergillus FumigatusFluorescent Reporter ConidiaInnate Immune ResponsePhagocytosisCell MigrationFungal Growth Inhibition

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