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Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus

Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus

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12:29 min

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April 19, 2017

DOI:

10.3791/55444-v

DOI:
10.3791/55444-v

•

12:29 min
April 19, 2017

•

2 Views
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1Aberdeen Fungal Group, MRC Centre for Medical Mycology, Institute of Medical Sciences,University of Aberdeen, 2Department of Medicine,Memorial Sloan-Kettering Cancer Center, New York, US

Summary

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Here, we describe a protocol to assess antifungal activity of primary human immune cells in real-time using fluorescent Aspergillus reporter conidia in conjunction with live-cell video microscopy and flow cytometry. Generated data provide insight into host cell-Aspergillus interactions such as fungicidal activity, phagocytosis, cell migration and inhibition of fungal growth.

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Live Cell ImagingAntifungal ActivityHuman NeutrophilsHuman MonocytesAspergillus FumigatusFluorescent Reporter ConidiaInnate Immune ResponsePhagocytosisCell MigrationFungal Growth Inhibition

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Cite this Article

Brunel, S. F., Bain, J. M., King, J., Heung, L. J., Kasahara, S., Hohl, T. M., Warris, A. Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus. J. Vis. Exp. (122), e55444, doi:10.3791/55444 (2017).

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