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Geliştirilmiş Numune Kombine Öncül İzotop Etiketleme ve Isobaric Etiketlemeyi Kullanma Dokuların çoğullama (cPILOT)
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
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Geliştirilmiş Numune Kombine Öncül İzotop Etiketleme ve Isobaric Etiketlemeyi Kullanma Dokuların çoğullama (cPILOT)

Türkçe

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6,749 Views

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09:06 min

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May 01, 2017

DOI:

10.3791/55406-v

DOI:
10.3791/55406-v

•

09:06 min
May 01, 2017

•

5 Views
, , , ,
1Department of Chemistry,University of Pittsburgh, 2SGS North America Inc., 3Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science,Janssen Research and Development

Summary

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Kombine ön-madde izotopik etiketleme ve izobarik etiketleme (cPILOT) izobarik etiketlerin örnek çoğullama kabiliyetini arttıran bir sayısal proteomik stratejisidir. Bu protokol bir Alzheimer hastalığı fare modeli ve vahşi tipli kontrollerden dokulara cPILOT uygulanmasını tarif etmektedir.

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Cite this Article

King, C. D., Dudenhoeffer, J. D., Gu, L., Evans, A. R., Robinson, R. A. S. Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT). J. Vis. Exp. (123), e55406, doi:10.3791/55406 (2017).

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