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Verbesserte Sample Multiplexing von Gewebe mit Combined Precursor Isotopenmarkierung und Isobaric Tagging (cPILOT)
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Biochemistry
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JoVE Journal Biochemistry
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
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Verbesserte Sample Multiplexing von Gewebe mit Combined Precursor Isotopenmarkierung und Isobaric Tagging (cPILOT)

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6,749 Views

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09:06 min

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May 01, 2017

DOI:

10.3791/55406-v

DOI:
10.3791/55406-v

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09:06 min
May 01, 2017

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5 Views
, , , ,
1Department of Chemistry,University of Pittsburgh, 2SGS North America Inc., 3Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science,Janssen Research and Development

Summary

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Kombinierte Vorläufer Isotopenmarkierung und isobar Tagging (cPILOT) ist eine quantitative Proteomik-Strategie, die Probe Multiplexierungsfähigkeiten von isobar-Tags verbessert. Dieses Protokoll beschreibt die Anwendung von cPILOT an Gewebe von einer Krankheit Mausmodell und Wildtyp-Kontrollen der Alzheimer.

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King, C. D., Dudenhoeffer, J. D., Gu, L., Evans, A. R., Robinson, R. A. S. Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT). J. Vis. Exp. (123), e55406, doi:10.3791/55406 (2017).

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