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Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
JoVE Journal
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JoVE Journal Biyokimya
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

6,749 Views

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09:06 min

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May 01, 2017

DOI:

10.3791/55406-v

DOI:
10.3791/55406-v

•

09:06 min
May 01, 2017

•

5 Views
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1Kimya Bölümü,University of Pittsburgh, 2SGS North America Inc., 3Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science,Janssen Research and Development

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Combined precursor isotopic labeling and isobaric tagging (cPILOT) is a quantitative proteomics strategy that enhances sample multiplexing capabilities of isobaric tags. This protocol describes the application of cPILOT to tissues from an Alzheimer's disease mouse model and wild-type controls.

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King, C. D., Dudenhoeffer, J. D., Gu, L., Evans, A. R., Robinson, R. A. S. Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT). J. Vis. Exp. (123), e55406, doi:10.3791/55406 (2017).

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