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Razionalizzato isolamento di singole cellule TCR e generazione di vettori retrovirali per In Vitro e In Vivo l'espressione del TCR umano
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11:21 min
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September 10, 2017
DOI:
1Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute,Baylor College of Medicine, Texas Children’s Hospital, 2Benaroya Research Institute at Virginia Mason, 3Center for Human Immunobiology,Baylor College of Medicine, Texas Children’s Hospital, 4Department of Pediatrics, Section of Diabetes and Endocrinology,Baylor College of Medicine, Texas Children’s Hospital
Summary
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I combines di protocollo corrente singola cella coppia umana TCR alfa e beta catena sequenziamento con snella generazione di vettori retrovirali compatibile con espressione TCR in vitro e in vivo .
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Sprouse, M. L., Blahnik, G., Lee, T., Tully, N., Benarjee, P., James, E. A., Redondo, M. J., Bettini, M. L., Bettini, M. Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs. J. Vis. Exp. (127), e55379, doi:10.3791/55379 (2017).
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