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Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
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Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
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11:21 min
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September 10, 2017
DOI:
1Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute,Baylor College of Medicine, Texas Children’s Hospital, 2Benaroya Research Institute at Virginia Mason, 3Center for Human Immunobiology,Baylor College of Medicine, Texas Children’s Hospital, 4Department of Pediatrics, Section of Diabetes and Endocrinology,Baylor College of Medicine, Texas Children’s Hospital
Summary
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The current protocol combines single cell paired human TCR alpha and beta chain sequencing with streamlined generation of retroviral vectors compatible with in vitro and in vivo TCR expression.
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Sprouse, M. L., Blahnik, G., Lee, T., Tully, N., Benarjee, P., James, E. A., Redondo, M. J., Bettini, M. L., Bettini, M. Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs. J. Vis. Exp. (127), e55379, doi:10.3791/55379 (2017).
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