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Gestroomlijnd eencellige TCR isolatie en generatie van retrovirale vectoren voor In Vitro en In Vivo expressie van menselijke TCRs
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September 10, 2017
DOI:
1Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute,Baylor College of Medicine, Texas Children’s Hospital, 2Benaroya Research Institute at Virginia Mason, 3Center for Human Immunobiology,Baylor College of Medicine, Texas Children’s Hospital, 4Department of Pediatrics, Section of Diabetes and Endocrinology,Baylor College of Medicine, Texas Children’s Hospital
Summary
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De huidige protocol combineert eencellige gekoppeld menselijke TCR alpha en beta keten sequencing met gestroomlijnd generatie van retrovirale vectoren compatibel met in vitro en in vivo TCR expressie.
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Sprouse, M. L., Blahnik, G., Lee, T., Tully, N., Benarjee, P., James, E. A., Redondo, M. J., Bettini, M. L., Bettini, M. Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs. J. Vis. Exp. (127), e55379, doi:10.3791/55379 (2017).
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