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Bioengineering
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细胞分化和牵引力的相关分析高通量细胞芯片平台
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
Please note that all translations are automatically generated.
Click here for the English version.
细胞分化和牵引力的相关分析高通量细胞芯片平台
DOI:
10.3791/55362-v
•
12:04 min
•
March 01, 2017
•
Kerim B. Kaylan
,
Andreas P. Kourouklis
,
Gregory H. Underhill
1
Department of Bioengineering
,
University of Illinois at Urbana–Champaign
Chapters
00:05
Title
01:05
Fabrication of Polyacrylamide Substrates
02:57
Fabrication of Arrays
05:15
Cell Culture and Assay Readout
09:06
Results: Biochemical and Biophysical Cues in Liver Progenitor Differentiation
10:58
Conclusion
Summary
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细胞分化是由微环境因素的影响,包括基质组合物和基材的材料性质的宿主调节。我们在这里描述利用细胞微阵列与牵引力显微镜一起同时评估细胞分化和生物力学细胞 - 基底相互作用的微环境上下文的功能的技术。
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