/
/
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
A subscription to JoVE is required to view this content. Sign in or start your free trial.
10,500 Views
•
09:26 min
•
March 26, 2017
DOI:
Summary
Automatically generated
This protocol describes the reconstitution of nucleosomes containing differentially isotope-labeled sister histones. At the same time, asymmetrically post-translationally modified nucleosomes can be generated after using a premodified histone copy. These preparations can be readily used to study modification crosstalk mechanisms, simultaneously on both sister histones, by using high-resolution NMR spectroscopy.
Read Article
Cite this Article
Liokatis, S. Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones. J. Vis. Exp. (121), e55349, doi:10.3791/55349 (2017).
Copy