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Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
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Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
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March 26, 2017
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Summary
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This protocol describes the reconstitution of nucleosomes containing differentially isotope-labeled sister histones. At the same time, asymmetrically post-translationally modified nucleosomes can be generated after using a premodified histone copy. These preparations can be readily used to study modification crosstalk mechanisms, simultaneously on both sister histones, by using high-resolution NMR spectroscopy.
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Liokatis, S. Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones. J. Vis. Exp. (121), e55349, doi:10.3791/55349 (2017).
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