Journal
/
/
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
Journal JoVE
Biochimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

10,500 Views

09:26 min

March 26, 2017

DOI:

09:26 min
March 26, 2017

7 Views

Summary

Automatically generated

This protocol describes the reconstitution of nucleosomes containing differentially isotope-labeled sister histones. At the same time, asymmetrically post-translationally modified nucleosomes can be generated after using a premodified histone copy. These preparations can be readily used to study modification crosstalk mechanisms, simultaneously on both sister histones, by using high-resolution NMR spectroscopy.

Read Article