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Developmental Biology

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Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy
 

Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy

Article DOI: 10.3791/55335-v 11:55 min February 24th, 2017
February 24th, 2017

章节

总结概括

Zebrafish retinal regeneration has mostly been studied using fixed retinas. However, dynamic processes such as interkinetic nuclear migration occur during the regenerative response and require live-cell imaging to investigate the underlying mechanisms. Here, we describe culture and imaging conditions to monitor Interkinetic Nuclear Migration (INM) in real-time using multiphoton microscopy.

Tags

Keywords: Retinal Explant Live-cell Imaging Multiphoton Microscopy Zebrafish Interkinetic Nuclear Migration Retinal Regeneration Muller Glia Microglial Behavior Phagocytosis Agarose FluoroDish
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