Journal
/
Biología del desarrollo
/
Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy

Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy

DOI:
10.3791/55335-v

•

11:55 min

•

February 24, 2017

•

, , ,
1Department of Biological Sciences,University of Notre Dame, 2Department of Neurosurgery,Mayo Clinic

Capítulos

  • 00:05Título
  • 01:11Isolation and Culturing of Retinal Explants
  • 03:30Multiphoton Microscopy
  • 07:52Analysis of Velocity
  • 09:34Results: Analysis of Apical and Basal Migration Velocities
  • 10:55Conclusion

Summary

Traducción Automática

Traducción Automática

Zebrafish retinal regeneration has mostly been studied using fixed retinas. However, dynamic processes such as interkinetic nuclear migration occur during the regenerative response and require live-cell imaging to investigate the underlying mechanisms. Here, we describe culture and imaging conditions to monitor Interkinetic Nuclear Migration (INM) in real-time using multiphoton microscopy.

Tags

Keywords: Retinal ExplantLive-cell ImagingMultiphoton MicroscopyZebrafishInterkinetic Nuclear MigrationRetinal RegenerationMuller GliaMicroglial BehaviorPhagocytosisAgaroseFluoroDish

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/55335?article_type=v

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury
check_url/es/55335?article_type=v

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation
check_url/es/55335?article_type=v

Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
check_url/es/55335?article_type=v

Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
check_url/es/55335?article_type=v

Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
check_url/es/55335?article_type=v

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning
check_url/es/55335?article_type=v

In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
check_url/es/55335?article_type=v

Live Imaging of Mouse Secondary Palate Fusion
check_url/es/55335?article_type=v

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
check_url/es/55335?article_type=v

Live Cell Imaging of Chromosome Segregation During Mitosis

Read Article