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Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

DOI:
10.3791/55286-v

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06:00 min

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May 08, 2017

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, ,
1Department of Agriculture, Nutrition, and Veterinary Sciences,University of Nevada, Reno, 2Department of Biochemistry and Molecular Biology,University of Nevada, Reno

Chapters

  • 00:05Title
  • 00:51Tick Housing and Collecting Immature Ticks
  • 01:46Infesting Mice with Immature Ticks
  • 03:34Collecting and Maintaining Engorged Ticks
  • 04:56Results: Tick Collection
  • 05:33Conclusion

Summary

Automatic Translation

Automatic Translation

The production of healthy laboratory-reared ticks is essential to studies on tick biology, and tick-pathogen interactions. Here we demonstrate a simple protocol for immature tick feeding that is cost-effective and less stressful to mice.

Tags

Ixodes ScapularisBlack-legged TickTick FeedingTick Colony RearingRodent HostTick MortalityTick VialsTick LarvaeTick NymphsMouse InfestationIsoflurane AnesthesiaPetroleum-jelly-based Ophthalmic Ointment

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