Journal
/
免疫与感染
/
Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

DOI:
10.3791/55286-v

•

06:00 min

•

May 08, 2017

•

, ,
1Department of Agriculture, Nutrition, and Veterinary Sciences,University of Nevada, Reno, 2Department of Biochemistry and Molecular Biology,University of Nevada, Reno

Chapters

  • 00:05Title
  • 00:51Tick Housing and Collecting Immature Ticks
  • 01:46Infesting Mice with Immature Ticks
  • 03:34Collecting and Maintaining Engorged Ticks
  • 04:56Results: Tick Collection
  • 05:33Conclusion

Summary

自动翻译

自动翻译

The production of healthy laboratory-reared ticks is essential to studies on tick biology, and tick-pathogen interactions. Here we demonstrate a simple protocol for immature tick feeding that is cost-effective and less stressful to mice.

Tags

Ixodes ScapularisBlack-legged TickTick FeedingTick Colony RearingRodent HostTick MortalityTick VialsTick LarvaeTick NymphsMouse InfestationIsoflurane AnesthesiaPetroleum-jelly-based Ophthalmic Ointment

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Phenotypic Analysis of Rodent Malaria Parasite Asexual and Sexual Blood Stages and Mosquito Stages

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System

Feeding and Quantifying Animal-Derived Blood and Artificial Meals in Aedes aegypti Mosquitoes

Standard Membrane Feeding Assay for the Detection of Plasmodium falciparum Infection in Anopheles Mosquito Vectors

Read Article