The production of healthy laboratory-reared ticks is essential to studies on tick biology, and tick-pathogen interactions. Here we demonstrate a simple protocol for immature tick feeding that is cost-effective and less stressful to mice.
Nuss, A. B., Mathew, M. G., Gulia-Nuss, M. Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice. J. Vis. Exp. (123), e55286, doi:10.3791/55286 (2017).