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Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

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10,757 Views

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08:52 min

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February 18, 2017

DOI:

10.3791/55284-v

DOI:
10.3791/55284-v

•

08:52 min
February 18, 2017

•

7 Views
, , , , ,
1Department of Medicine II,Saarland University Medical Center, 2Department of Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM),University of Saarland

Summary

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Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.

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Julich-Haertel, H., Tiwari, M., Mehrfeld, C., Krause, E., Kornek, M., Lukacs-Kornek, V. Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination. J. Vis. Exp. (120), e55284, doi:10.3791/55284 (2017).

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