Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.
Julich-Haertel, H., Tiwari, M., Mehrfeld, C., Krause, E., Kornek, M., Lukacs-Kornek, V. Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination. J. Vis. Exp. (120), e55284, doi:10.3791/55284 (2017).