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Developmental Biology

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JoVE Journal Developmental Biology
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
 

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Article DOI: 10.3791/55284-v • 08:52 min • February 18th, 2017
February 18th, 2017 •
1, 1, 1, 2, 1, 1
1Department of Medicine II, Saarland University Medical Center, 2Department of Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), University of Saarland

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Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.

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Keywords: Liver Progenitor Cells Liver Digestion Protocol Flow Cytometry Surface Marker Combination Cell Isolation Cell Biology Hematopoietic Cells Endothelial Cells Mouse Liver Tissue Dissociation Cell Suspension Cell Counting Propidium Iodide
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