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Hücre içi Boyama ve Sitometrisi Hipertansiyon Modelinde Fare Aort, Böbrek ve Lenf düğümleri içinde lenfosit alt tanımak
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Immunology and Infection
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JoVE Journal Immunology and Infection
Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension
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Hücre içi Boyama ve Sitometrisi Hipertansiyon Modelinde Fare Aort, Böbrek ve Lenf düğümleri içinde lenfosit alt tanımak

Türkçe

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17,926 Views

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09:20 min

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January 28, 2017

DOI:

10.3791/55266-v

DOI:
10.3791/55266-v

•

09:20 min
January 28, 2017

•

10 Views
, , ,
1Department of Medicine, Division of Clinical Pharmacology,Vanderbilt University Medical Center, 2Department of Molecular Physiology and Biophysics,Vanderbilt University, 3Department of Pharmacology and Toxicology, Faculty of Pharmacy,Mansoura University

Summary

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Bu makalede, tanımlamak ve hücre içi boyama ile fare böbrek, aort ve lenf düğümleri içinde mevcut fonksiyonel T lenfosit alt grupları ölçmek ve akım sitometri ayrıntılı bir metodoloji sağlar. anjiotensin II hipertansiyon modeli açıklamak adım-adım, usul ve flow sitometri temel ilkelerine ve hücre içi boyama seçildi.

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Intracellular StainingFlow CytometryLymphocyte SubsetsMurine AortaKidneyLymph NodesHypertensionAngiotensin IISingle-cell SuspensionImmune CellsCardiovascular DiseasePerfusionDissectionDensity Gradient Centrifugation

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Laroumanie, F., Dale, B. L., Saleh, M. A., Madhur, M. S. Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension. J. Vis. Exp. (119), e55266, doi:10.3791/55266 (2017).

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