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Immunology and Infection

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JoVE Journal Immunology and Infection
Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension
 

Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension

Article DOI: 10.3791/55266-v • 09:20 min • January 28th, 2017
January 28th, 2017 •
1, 2, 3, 1
1Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center, 2Department of Molecular Physiology and Biophysics, Vanderbilt University, 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University

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This article provides detailed methodology to identify and quantify functional T lymphocyte subsets present within murine kidney, aorta and lymph nodes by intracellular staining and flow cytometry. The model of angiotensin II induced hypertension was chosen to explain, step-by-step, the procedures and fundamental principles of flow cytometry and intracellular staining.

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Keywords: Intracellular Staining Flow Cytometry Lymphocyte Subsets Murine Aorta Kidney Lymph Nodes Hypertension Angiotensin II Single-cell Suspension Immune Cells Cardiovascular Disease Perfusion Dissection Tissue Dissociation Density Gradient Centrifugation
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