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Intracellulaire kleuring en flowcytometrie om lymfocyten in muizen Aorta, nieren en lymfeklieren te identificeren in een Model of Hypertension
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Immunology and Infection
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JoVE Journal Immunology and Infection
Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension
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Intracellulaire kleuring en flowcytometrie om lymfocyten in muizen Aorta, nieren en lymfeklieren te identificeren in een Model of Hypertension

Nederlands

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17,926 Views

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09:20 min

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January 28, 2017

DOI:

10.3791/55266-v

DOI:
10.3791/55266-v

•

09:20 min
January 28, 2017

•

10 Views
, , ,
1Department of Medicine, Division of Clinical Pharmacology,Vanderbilt University Medical Center, 2Department of Molecular Physiology and Biophysics,Vanderbilt University, 3Department of Pharmacology and Toxicology, Faculty of Pharmacy,Mansoura University

Summary

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Dit artikel bevat gedetailleerde methodologie voor het identificeren en kwantificeren van functionele T-lymfocyten subsets in muizen nieren, aorta en de lymfeklieren aanwezig door intracellulaire kleuring en flowcytometrie. Het model van angiotensine II geïnduceerde hypertensie werd gekozen uitleggen, stap voor stap, de procedures en de grondbeginselen van flowcytometrie en intracellulaire kleuring.

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Laroumanie, F., Dale, B. L., Saleh, M. A., Madhur, M. S. Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension. J. Vis. Exp. (119), e55266, doi:10.3791/55266 (2017).

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