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Diferenciação eficiente dos Pluripotent Stem Cells para NKX6-1<sup> +</sup> Progenitores pancreáticas
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Developmental Biology
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JoVE Journal Developmental Biology
Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

Diferenciação eficiente dos Pluripotent Stem Cells para NKX6-1<sup> +</sup> Progenitores pancreáticas

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7,748 Views

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09:23 min

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March 07, 2017

DOI:

10.3791/55265-v

DOI:
10.3791/55265-v

•

09:23 min
March 07, 2017

•

1 Views
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1Toronto General Research Institute,University Health Network, 2McEwen Centre for Regenerative Medicine,University Health Network, 3Department of Physiology,University of Toronto

Summary

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Aqui nós descrevemos um protocolo de 4 estágios para diferenciar as células-tronco embrionárias humanas para NKX6-1 + progenitoras pancreáticas in vitro. Este protocolo pode ser aplicado a uma variedade de linhas de células estaminais pluripotentes humanas.

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Cite this Article

McGaugh, E. C., Nostro, M. C. Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors. J. Vis. Exp. (121), e55265, doi:10.3791/55265 (2017).

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