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Effiziente Differenzierung von pluripotenten Stammzellen zu NKX6-1<sup> +</sup> Pancreatic Vorläufern
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Developmental Biology
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JoVE Journal Developmental Biology
Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
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Effiziente Differenzierung von pluripotenten Stammzellen zu NKX6-1<sup> +</sup> Pancreatic Vorläufern

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7,748 Views

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09:23 min

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March 07, 2017

DOI:

10.3791/55265-v

DOI:
10.3791/55265-v

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09:23 min
March 07, 2017

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1 Views
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1Toronto General Research Institute,University Health Network, 2McEwen Centre for Regenerative Medicine,University Health Network, 3Department of Physiology,University of Toronto

Summary

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Hier beschreiben wir eine 4-Stufen - Protokoll an humanen embryonalen Stammzellen zu NKX6-1 + Pankreas - Vorläufern in vitro zu differenzieren. Dieses Protokoll kann auf einer Vielzahl von menschlichen pluripotenten Stammzelllinien angewendet werden.

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McGaugh, E. C., Nostro, M. C. Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors. J. Vis. Exp. (121), e55265, doi:10.3791/55265 (2017).

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