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Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

7,748 Views

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09:23 min

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March 07, 2017

DOI:

10.3791/55265-v

DOI:
10.3791/55265-v

•

09:23 min
March 07, 2017

•

1 Views
,
1Toronto General Research Institute,University Health Network, 2McEwen Centre for Regenerative Medicine,University Health Network, 3Department of Physiology,University of Toronto

Summary

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Here we describe a 4-stage protocol to differentiate human embryonic stem cells to NKX6-1+ pancreatic progenitors in vitro. This protocol can be applied to a variety of human pluripotent stem cell lines.

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Cite this Article

McGaugh, E. C., Nostro, M. C. Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors. J. Vis. Exp. (121), e55265, doi:10.3791/55265 (2017).

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