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Aislamiento y caracterización de las células mesenquimales del estroma de cordón umbilical humano y fetal Placenta
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
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Aislamiento y caracterización de las células mesenquimales del estroma de cordón umbilical humano y fetal Placenta

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45,197 Views

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07:06 min

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April 03, 2017

DOI:

10.3791/55224-v

DOI:
10.3791/55224-v

•

07:06 min
April 03, 2017

•

65 Views
, , , , , ,
1Department of Biological Sciences,Oakland University, 2OU-WB Institute for Stem Cell and Regenerative Medicine, 3Department of Obstetrics and Gynecology,St. John Provindence – Providence Park Hospital, 4Department of Neurosurgery,Beaumont Health System

Summary

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Aquí, se presenta un protocolo para la disección de cordón umbilical humano umbilical (CU) y la muestra de placenta fetal en cable de forro (CL), gelatina de Wharton (WJ), la unión de cordón placenta (CPJ), y placenta fetal (FP) para el aislamiento y caracterización de células estromales mesenquimales (MSCs) utilizando la técnica de cultivo de explantes.

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Cite this Article

Beeravolu, N., McKee, C., Alamri, A., Mikhael, S., Brown, C., Perez-Cruet, M., Chaudhry, G. R. Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta. J. Vis. Exp. (122), e55224, doi:10.3791/55224 (2017).

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