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Developmental Biology

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JoVE Journal Developmental Biology
Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
 

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

Article DOI: 10.3791/55224-v • 07:06 min • April 3rd, 2017
April 3rd, 2017 •
1,2, 1,2, 1,2, 3, 1,2, 2,4, 1,2
1Department of Biological Sciences, Oakland University, 2OU-WB Institute for Stem Cell and Regenerative Medicine, 3Department of Obstetrics and Gynecology, St. John Provindence - Providence Park Hospital, 4Department of Neurosurgery, Beaumont Health System

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Here, we present a protocol for the dissection of human umbilical cord (UC) and fetal placenta sample into cord lining (CL), Wharton's jelly (WJ), cord-placenta junction (CPJ), and fetal placenta (FP) for the isolation and characterization of mesenchymal stromal cells (MSCs) using the explant culture technique.

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Isolation Characterization Mesenchymal Stromal Cells Human Umbilical Cord Fetal Placenta Perinatal Tissue Sources Umbilical Cord Lining Wharton's Jelly Cord-placenta Junction Stem Cell Biology Alternative Medicine High Quality Cell Populations Multi-potent Cord Mesenchymal Stromal Cells Diseases And Disorders Adult Tissues Specific Niches Petri Dish Bio Safety Cabinet Needle And Syringe Ice Cold PBS
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