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Isolatie en karakterisering van mesenchymale stromale cellen uit menselijke navelstreng en foetale Placenta
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
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Isolatie en karakterisering van mesenchymale stromale cellen uit menselijke navelstreng en foetale Placenta

Nederlands

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45,185 Views

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07:06 min

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April 03, 2017

DOI:

10.3791/55224-v

DOI:
10.3791/55224-v

•

07:06 min
April 03, 2017

•

65 Views
, , , , , ,
1Department of Biological Sciences,Oakland University, 2OU-WB Institute for Stem Cell and Regenerative Medicine, 3Department of Obstetrics and Gynecology,St. John Provindence – Providence Park Hospital, 4Department of Neurosurgery,Beaumont Health System

Summary

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Hier presenteren we een protocol voor de ontleding van menselijke navelstreng (UC) en foetale placenta monster in koord bekleding (CL), Wharton's gelei (WJ), cord-placenta junctie (CPJ) en foetale placenta (FP) voor het isoleren en karakterisering van mesenchymale stromale cellen (MSC's) met de explantaatkweek techniek.

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Cite this Article

Beeravolu, N., McKee, C., Alamri, A., Mikhael, S., Brown, C., Perez-Cruet, M., Chaudhry, G. R. Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta. J. Vis. Exp. (122), e55224, doi:10.3791/55224 (2017).

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