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単一細胞のシーケンシングを使用したコピー数変化の検出

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Detection of Copy Number Alterations Using Single Cell Sequencing

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単一細胞のシーケンシングを使用したコピー数変化の検出

11,256 Views

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09:45 min

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February 17, 2017

DOI:

10.3791/55143-v

DOI:

10.3791/55143-v

•

09:45 min

February 17, 2017

•

9 Views

Kristin A. Knouse^1,2,3, Jie Wu⁴, Austin Hendricks⁵

¹Koch Institute for Integrative Cancer Research, Department of Biology,Massachusetts Institute of Technology, ²Howard Hughes Medical Institute, ³Division of Health Sciences and Technology,Harvard Medical School, ⁴The Barbara K. Ostrom (1978) Bioinformatics and Computing Facility in the Swanson Biotechnology Center, Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, ⁵BioMicro Center, Department of Biology,Massachusetts Institute of Technology

Summary

単一細胞配列決定は、高解像度でゲノムの変化に対処するため、ますます人気とアクセス可能なツールです。我々は、単一の細胞内のコピー数の変化を識別するために、単一細胞の配列決定を使用するプロトコルを提供します。

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